Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-CLIC1 Antibody in A375 cell line lysates (35ug/lane). CLIC1(arrow) was detected using the purified Pab (1:60dilution).| Product Name | CLIC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLIC1; G6; NCC27; Chloride intracellular channel protein 1; Chloride channel ABP; Nuclear chloride ion channel 27; Regulatory nuclear chloride ion channel protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G6 NCC27 |
|---|---|
| Alternative Names | CLIC1 G6 NCC27 Chloride intracellular channel protein 1 Chloride channel ABP Nuclear chloride ion channel 27 Regulatory nuclear chloride ion channel protein |
| Molecular Weight(MW) | 26923 Da |
| Function | Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle |
| Tissue Specificity | Expression is prominent in heart, placenta, liver, kidney and pancreas |
| Cellular Localization | Nucleus. Nucleus membrane; Single-pass membrane protein (Probable). Cytoplasm. Cell membrane; Single-pass membrane protein (Probable). Note=Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain |
| Entrez Gene | 1192 |
|---|
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-CLIC1 Antibody in A375 cell line lysates (35ug/lane). CLIC1(arrow) was detected using the purified Pab (1:60dilution).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
