Application
Western blot analysis of CLIC4 (arrow) using rabbit polyclonal CLIC4 Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CLIC4 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CLIC4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CLIC4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLIC4; Chloride intracellular channel protein 4; Intracellular chloride ion channel protein p64H1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CLIC4 Chloride intracellular channel protein 4 Intracellular chloride ion channel protein p64H1 |
|---|---|
| Molecular Weight(MW) | 28772 Da |
| Function | Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis) |
| Tissue Specificity | Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Cytoplasmic vesicle membrane; Single-pass membrane protein (Probable). Nucleus matrix. Cell membrane; Single-pass membrane protein (Probable). Mitochondrion. Cell junction. Note=Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles |
| Entrez Gene | 25932 |
|---|
Application
Western blot analysis of CLIC4 (arrow) using rabbit polyclonal CLIC4 Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CLIC4 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CLIC4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
