Application
Western blot analysis of CLIP1 Antibody (N-term) in Hela cell line lysates (35ug/lane). CLIP1 (arrow) was detected using the purified Pab.Application
Western blot analysis of CLIP1 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). CLIP1 (arrow) was detected using the purified Pab.Application
CLIP1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLIP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CLIP1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CLIP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB,IHC,FC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYLN1, RSN |
|---|---|
| Alternative Names | CLIP1 CYLN1 RSN CAP-Gly domain-containing linker protein 1 Cytoplasmic linker protein 1 Cytoplasmic linker protein 170 alpha-2 Reed-Sternberg intermediate filament-associated protein Restin |
| Molecular Weight(MW) | 162246 Da |
| Function | Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking |
| Tissue Specificity | Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle. Note=Associated with the cytoskeleton. Detected at the plus ends of microtubules in the cytosol, and close to plasma membrane ruffles. Associates with the membranes of intermediate macropinocytic vesicles |
| Entrez Gene | 6249 |
|---|
Application
Western blot analysis of CLIP1 Antibody (N-term) in Hela cell line lysates (35ug/lane). CLIP1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of CLIP1 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). CLIP1 (arrow) was detected using the purified Pab.
Application
CLIP1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLIP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CLIP1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
