Application
Western blot analysis of CLNS1A antibody (C-term) (Cat.# AP6520b) in HL60 cell line lysates (35ug/lane). CLNS1A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of hela cells using CLNS1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CLNS1A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLNS1A; CLCI; ICLN; Methylosome subunit pICln; Chloride channel, nucleotide sensitive 1A; Chloride conductance regulatory protein ICln; Chloride ion current inducer protein; Reticulocyte pICln |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLCI ICLN |
|---|---|
| Alternative Names | CLNS1A CLCI ICLN Methylosome subunit pICln Chloride channel nucleotide sensitive 1A Chloride conductance regulatory protein ICln Chloride ion current inducer protein Reticulocyte pICln |
| Molecular Weight(MW) | 26215 Da |
| Function | The interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway |
| Tissue Specificity | This CLNS1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195~225 amino acids from the C-terminal region of human CLNS1A. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Note=A small fraction is also associated with the cytoskeleton |
| Entrez Gene | 1207 |
|---|
Application
Western blot analysis of CLNS1A antibody (C-term) (Cat.# AP6520b) in HL60 cell line lysates (35ug/lane). CLNS1A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of hela cells using CLNS1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
