Application
CLP1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CLP1 antibody detected the CLP1 protein (arrow).| Product Name | CLP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLP1; HEAB; Polyribonucleotide 5'-hydroxyl-kinase Clp1; Polynucleotide kinase Clp1; Pre-mRNA cleavage complex II protein Clp1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HEAB |
|---|---|
| Alternative Names | CLP1 HEAB Polyribonucleotide 5'-hydroxyl-kinase Clp1 Polynucleotide kinase Clp1 Pre-mRNA cleavage complex II protein Clp1 |
| Molecular Weight(MW) | 47646 Da |
| Function | Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA- induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing |
| Tissue Specificity | This CLP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-336 amino acids from the C-terminal region of human CLP1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 10978 |
|---|---|
| Nucleotide Accession | NP_006822.1 NP_001136069.1 |
Application
CLP1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CLP1 antibody detected the CLP1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
