Application
Western blot analysis of CLTB Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).CLTB (arrow) was detected using the purified Pab.Application
CLTB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLTB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of CLTB (arrow) using rabbit polyclonal CLTB Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTB gene.Application
CLTB Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CLTB Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLTB; Clathrin light chain B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CLTB Clathrin light chain B |
|---|---|
| Molecular Weight(MW) | 25190 Da |
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles |
| Tissue Specificity | This CLTB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-130 amino acids from the Central region of human CLTB. |
| Cellular Localization | Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic face of coated pits and vesicles |
| Entrez Gene | 1212 |
|---|
Application
Western blot analysis of CLTB Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).CLTB (arrow) was detected using the purified Pab.
Application
CLTB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLTB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of CLTB (arrow) using rabbit polyclonal CLTB Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTB gene.
Application
CLTB Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
