Application
CLTC Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CLTC antibody detected the CLTC protein (arrow).Application
CLTC antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLTC antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CLTC Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CLTC Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLTC; CLH17; CLTCL2; KIAA0034; Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLH17 CLTCL2 KIAA0034 |
|---|---|
| Alternative Names | CLTC CLH17 CLTCL2 KIAA0034 Clathrin heavy chain 1 Clathrin heavy chain on chromosome 17 |
| Molecular Weight(MW) | 191615 Da |
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network |
| Tissue Specificity | This CLTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1024-1054 amino acids from the Central region of human CLTC. |
| Cellular Localization | Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 1213 |
|---|---|
| Nucleotide Accession | NP_004850.1 |
Application
CLTC Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CLTC antibody detected the CLTC protein (arrow).
Application
CLTC antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLTC antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CLTC Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
