Application
CMGA Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CMGA antibody detected the CMGA protein (arrow).Application
CMGA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CMGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of CMGA Antibody (C-term)(Cat#AP11784b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
CMGA Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CMGA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHGA; SS-18; WA-8; WE-14; LF-19; AL-11; GV-19; GR-44; Chromogranin-A; Pituitary secretory protein I; Vasostatin-1; Vasostatin I; Vasostatin-2; Vasostatin II; EA-92; ES-43; Pancreastatin; ER-37 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CHGA SS-18 WA-8 WE-14 LF-19 AL-11 GV-19 GR-44 Chromogranin-A Pituitary secretory protein I Vasostatin-1 Vasostatin I Vasostatin-2 Vasostatin II EA-92 ES-43 Pancreastatin ER-37 |
|---|---|
| Molecular Weight(MW) | 50688 Da |
| Function | Pancreastatin strongly inhibits glucose induced insulin release from the pancreas |
| Tissue Specificity | This CMGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-412 amino acids from the C-terminal region of human CMGA. |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle lumen (By similarity). Cytoplasmic vesicle, secretory vesicle membrane (By similarity). Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions (By similarity) |
| Entrez Gene | 1113 |
|---|---|
| Nucleotide Accession | NP_001266.1 |
Application
CMGA Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CMGA antibody detected the CMGA protein (arrow).
Application
CMGA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CMGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of CMGA Antibody (C-term)(Cat#AP11784b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
CMGA Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
