Application
Western blot analysis of CNN2 Antibody (Center) in mouse lung tissue lysates (35ug/lane). CNN2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CNN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CNN2 Antibody (Center) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CNN2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CNN2; Calponin-2; Calponin H2, smooth muscle; Neutral calponin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CNN2 Calponin-2 Calponin H2 smooth muscle Neutral calponin |
|---|---|
| Molecular Weight(MW) | 33697 Da |
| Function | Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity |
| Tissue Specificity | Heart and smooth muscle. |
| Entrez Gene | 1265 |
|---|
Application
Western blot analysis of CNN2 Antibody (Center) in mouse lung tissue lysates (35ug/lane). CNN2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CNN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CNN2 Antibody (Center) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
