Application
CNIH2 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CNIH2 antibody detected the CNIH2 protein (arrow).Application
CNIH2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CNIH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CNIH2 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of CNIH2 Antibody (N-term)(Cat#AP11529a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CNIH2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CNIH2; CNIL; Protein cornichon homolog 2; Cornichon-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CNIL |
|---|---|
| Alternative Names | CNIH2 CNIL Protein cornichon homolog 2 Cornichon-like protein |
| Molecular Weight(MW) | 18931 Da |
| Function | Regulates the trafficking and gating properties of AMPA- selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by regulating their rates of activation, deactivation and desensitization. Blocks CACNG8-mediated resensitization of AMPA receptors |
| Tissue Specificity | This CNIH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human CNIH2. |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein (By similarity). Cell projection, dendrite (By similarity). Cell projection, dendritic spine (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Note=Also localizes to the cell membrane of extrasynaptic sites (dendritic shafts, spines of pyramidal cells) (By similarity) |
| Entrez Gene | 254263 |
|---|---|
| Nucleotide Accession | NP_872359.1 |
Application
CNIH2 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CNIH2 antibody detected the CNIH2 protein (arrow).
Application
CNIH2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CNIH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CNIH2 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of CNIH2 Antibody (N-term)(Cat#AP11529a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
