Application
Western blot analysis of CNOT8 Antibody (C-term) in CEM cell line lysates (35ug/lane). CNOT8 (arrow) was detected using the purified Pab.Application
Western blot analysis of CNOT8 (arrow) using rabbit polyclonal CNOT8 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CNOT8 gene.Application
Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CNOT8 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CNOT8; CALIF; POP2; CCR4-NOT transcription complex subunit 8; CAF1-like protein; CAF2; CCR4-associated factor 8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CALIF POP2 |
|---|---|
| Alternative Names | CNOT8 CALIF POP2 CCR4-NOT transcription complex subunit 8 CAF1-like protein CAF2 CCR4-associated factor 8 |
| Molecular Weight(MW) | 33540 Da |
| Function | Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex |
| Tissue Specificity | This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234~264 amino acids from the C-terminal region of human CNOT8. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 9337 |
|---|
Application
Western blot analysis of CNOT8 Antibody (C-term) in CEM cell line lysates (35ug/lane). CNOT8 (arrow) was detected using the purified Pab.
Application
Western blot analysis of CNOT8 (arrow) using rabbit polyclonal CNOT8 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CNOT8 gene.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
