Application
COASY Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the COASY antibody detected the COASY protein (arrow).Application
COASY antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COASY antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
COASY Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | COASY Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COASY; Bifunctional coenzyme A synthase; NBP; POV-2; Phosphopantetheine adenylyltransferase; Dephospho-CoA pyrophosphorylase; Pantetheine-phosphate adenylyltransferase; Dephospho-CoA kinase; Dephosphocoenzyme A kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | COASY Bifunctional coenzyme A synthase NBP POV-2 Phosphopantetheine adenylyltransferase Dephospho-CoA pyrophosphorylase Pantetheine-phosphate adenylyltransferase Dephospho-CoA kinase Dephosphocoenzyme A kinase |
|---|---|
| Molecular Weight(MW) | 62329 Da |
| Function | Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation |
| Tissue Specificity | Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 80347 |
|---|---|
| Nucleotide Accession | NP_001035995.1 NP_001035997.2 |
Application
COASY Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the COASY antibody detected the COASY protein (arrow).
Application
COASY antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COASY antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
COASY Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
