Application
Western blot analysis of COL17A1 Antibody (Center) in A375, A2058, HL-60, MCF-7 cell line lysates (35ug/lane). COL17A1 (arrow) was detected using the purified Pab.Application
COL17A1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL17A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
COL17A1 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | COL17A1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COL17A1; BP180; BPAG2; Collagen alpha-1(XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; 120 kDa linear IgA disease antigen; 120 kDa linear IgA dermatosis antigen; Linear IgA disease antigen 1; 97 kDa linear IgA disease anti |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BP180 BPAG2 |
|---|---|
| Alternative Names | COL17A1 BP180 BPAG2 Collagen alpha-1(XVII) chain 180 kDa bullous pemphigoid antigen 2 Bullous pemphigoid antigen 2 120 kDa linear IgA disease antigen 120 kDa linear IgA dermatosis antigen Linear IgA disease antigen 1 97 kDa linear IgA disease anti |
| Molecular Weight(MW) | 150419 Da |
| Function | May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane |
| Tissue Specificity | Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves |
| Cellular Localization | Cell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Note=Localized along the plasma membrane of the hemidesmosome 97 kDa linear IgA disease antigen: Secreted, extracellular space, extracellular matrix, basement membrane Note=Localized in the lamina lucida beneath the hemidesmosomes |
| Entrez Gene | 1308 |
|---|
Application
Western blot analysis of COL17A1 Antibody (Center) in A375, A2058, HL-60, MCF-7 cell line lysates (35ug/lane). COL17A1 (arrow) was detected using the purified Pab.
Application
COL17A1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL17A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
COL17A1 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
