Western blot analysis of GJB4 (arrow) using rabbit polyclonal GJB4 Antibody (H105.Connexin) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GJB4 gene.
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell
Tissue Specificity
This Connexin 30.3 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98~127 amino acids from the N-terminal region of human GJB4.
Cellular Localization
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction
Western blot analysis of GJB4 (arrow) using rabbit polyclonal GJB4 Antibody (H105.Connexin) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GJB4 gene.
Application Notes
WB:1:50~100
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.