Application
Western blot analysis of COMT (arrow) using rabbit polyclonal COMT Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the COMT gene (Lane 2) .Application
COMT antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COMT antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
COMT Antibody (N-term) western blot analysis in K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the COMT antibody detected the COMT protein (arrow).| Product Name | COMT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COMT; Catechol O-methyltransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | COMT Catechol O-methyltransferase |
|---|---|
| Molecular Weight(MW) | 30037 Da |
| Function | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol |
| Tissue Specificity | Brain, liver, placenta, lymphocytes and erythrocytes |
| Cellular Localization | Isoform Soluble: Cytoplasm. |
| Entrez Gene | 1312 |
|---|---|
| Nucleotide Accession | NP_001128634.1 |
Application
Western blot analysis of COMT (arrow) using rabbit polyclonal COMT Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the COMT gene (Lane 2) .
Application
COMT antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COMT antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
COMT Antibody (N-term) western blot analysis in K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the COMT antibody detected the COMT protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
