Application
A. Western blot analysis on HeLa cell lysate using anti-Cortactin RabMAb (Cat. #AJ1194a), dilution 1:100,000.Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Cortactin RabMAb (Cat. #AJ1194a).Application
C. Immunofluorescent staining of MCF7 cells using anti-Cortactin RabMAb (Cat. #AJ1194a).| Product Name | Cortactin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTTN, EMS1, Src substrate cortactin, Amplaxin;Oncogene EMS1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1922Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EMS1 |
|---|---|
| Alternative Names | CTTN EMS1 Src substrate cortactin Amplaxin Oncogene EMS1 |
| Molecular Weight(MW) | 61586 Da |
| Function | Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human Cortactin was used as an immunogen. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Note=Associated with membrane ruffles and lamellipodia |
| Entrez Gene | 2017 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-Cortactin RabMAb (Cat. #AJ1194a), dilution 1:100,000.
Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Cortactin RabMAb (Cat. #AJ1194a).
Application
C. Immunofluorescent staining of MCF7 cells using anti-Cortactin RabMAb (Cat. #AJ1194a).| Application Notes | WB:1:20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
