Application
Western blot analysis of COTL1 Antibody (C-term) in Hela cell line lysates (35ug/lane). COTL1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with COTL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
COTL1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of COTL1 (arrow) using rabbit polyclonal COTL1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COTL1 gene.| Product Name | COTL1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COTL1; CLP; Coactosin-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLP |
|---|---|
| Alternative Names | COTL1 CLP Coactosin-like protein |
| Molecular Weight(MW) | 15945 Da |
| Function | Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis |
| Tissue Specificity | Widely expressed with highest levels in placenta, lung, kidney and peripheral blood leukocytes and lower levels in brain, liver and pancreas |
| Cellular Localization | Cytoplasm, cytoskeleton (By similarity). Nucleus membrane; Peripheral membrane protein |
Application
Western blot analysis of COTL1 Antibody (C-term) in Hela cell line lysates (35ug/lane). COTL1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with COTL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
COTL1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of COTL1 (arrow) using rabbit polyclonal COTL1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COTL1 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
