Application
CPEB1 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the CPEB1 antibody detected the CPEB1 protein (arrow).| Product Name | CPEB1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CPEB1; CPEB; Cytoplasmic polyadenylation element-binding protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPEB |
|---|---|
| Alternative Names | CPEB1 CPEB Cytoplasmic polyadenylation element-binding protein 1 |
| Molecular Weight(MW) | 62595 Da |
| Function | Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU- 3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity) Induces the assembly of stress granules in the absence of stress |
| Tissue Specificity | Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines |
| Cellular Localization | Cytoplasm, P-body. Cytoplasmic granule. Cell junction, synapse. Membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell projection, dendrite (By similarity). Note=Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity) Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity) Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA, SYP, etc.) including CPE-containing RNAs (By similarity) |
| Entrez Gene | 64506 |
|---|---|
| Nucleotide Accession | NP_001073001.1 NP_001073002.1 |
Application
CPEB1 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the CPEB1 antibody detected the CPEB1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
