Application
CPSF3 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the CPSF3 antibody detected the CPSF3 protein (arrow).| Product Name | CPSF3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CPSF3; CPSF73; Cleavage and polyadenylation specificity factor subunit 3; Cleavage and polyadenylation specificity factor 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPSF73 |
|---|---|
| Alternative Names | CPSF3 CPSF73 Cleavage and polyadenylation specificity factor subunit 3 Cleavage and polyadenylation specificity factor 73 kDa subunit mRNA 3'-end-processing endonuclease CPSF-73 |
| Molecular Weight(MW) | 77486 Da |
| Function | Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner |
| Tissue Specificity | This CPSF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-221 amino acids from the Central region of human CPSF3. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 51692 |
|---|---|
| Nucleotide Accession | NP_057291.1 |
Application
CPSF3 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the CPSF3 antibody detected the CPSF3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
