Application
A. Western blot analysis on A431 cell lysate using anti-CREB RabMAb (Cat. #AJ1199a), dilution 1:1,000.Application
B. Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-CREB RabMAb (Cat. #AJ1199a).Application
C. Immunofluorescent staining of PC-12 cells using anti-CREB RabMAb (Cat. #AJ1199a).| Product Name | Creb Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CREB1, Cyclic AMP-responsive element-binding protein 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E306 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1: 5000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CREB1 Cyclic AMP-responsive element-binding protein 1 |
|---|---|
| Molecular Weight(MW) | 36688 Da |
| Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells |
| Tissue Specificity | A synthetic peptide corresponding to residues before DNA binding motif of human CREB was used as immunogen. This antibody can also recognize the splice isoform of CREB. Predicted to cross-react with bovine, based on sequence homology. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1385 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-CREB RabMAb (Cat. #AJ1199a), dilution 1:1,000.
Application
B. Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-CREB RabMAb (Cat. #AJ1199a).
Application
C. Immunofluorescent staining of PC-12 cells using anti-CREB RabMAb (Cat. #AJ1199a).| Application Notes | WB:1: 5000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
