Application
A. Immunohistochemical staining of untreated paraffin-embedded thyroid gland adenocarcinoma using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b).Application
B. Immunohistochemical staining of Phosphatase-treated (D) paraffin-embedded thyroid gland adenocarcinoma using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b).Application
C. Western blot analysis on A431 cell lysate using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b), 1:500 dilution. Cells were either (1) untreated or (2) treated with Forskolin.Application
D. Immunofluorescent staining of untreated A431 cells using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b).Application
E. Immunofluorescent staining of Phosphatase-treated A431 cells using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b).| Product Name | Creb Antibody Phospho (pS133) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CREB1, Cyclic AMP-responsive element-binding protein 1 |
| Modification | p-S133 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E113 |
| Host Species | Rabbit |
| Tested Applications | IHCWBIF |
| IHC:1:250 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CREB1 Cyclic AMP-responsive element-binding protein 1 |
|---|---|
| Molecular Weight(MW) | 36688 Da |
| Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB was used as immunogen. The antibody only detects CREB phosphorylated on Serine 133. Predicted to cross-react with bovine, chicken, and zebra fish, based on sequence homology. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1385 |
|---|
Application
A. Immunohistochemical staining of untreated paraffin-embedded thyroid gland adenocarcinoma using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b).
Application
B. Immunohistochemical staining of Phosphatase-treated (D) paraffin-embedded thyroid gland adenocarcinoma using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b).
Application
C. Western blot analysis on A431 cell lysate using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b), 1:500 dilution. Cells were either (1) untreated or (2) treated with Forskolin.
Application
D. Immunofluorescent staining of untreated A431 cells using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b).
Application
E. Immunofluorescent staining of Phosphatase-treated A431 cells using anti-Phospho-CREB (pS133) RabMAb (Cat. #AJ1199b).| Application Notes | IHC:1:250 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
