Application
Western blot analysis of CREB1 (arrow) using rabbit polyclonal CREB1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CREB1 gene.Application
CREB1 Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the CREB1 antibody detected the CREB1 protein (arrow).Application
CREB1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of CREB1 Antibody (Center)(Cat#AP11707c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | CREB1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CREB1; Cyclic AMP-responsive element-binding protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CREB1 Cyclic AMP-responsive element-binding protein 1 |
|---|---|
| Molecular Weight(MW) | 36688 Da |
| Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells |
| Tissue Specificity | This CREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the Central region of human CREB1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1385 |
|---|---|
| Nucleotide Accession | NP_004370.1 |
Application
Western blot analysis of CREB1 (arrow) using rabbit polyclonal CREB1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CREB1 gene.
Application
CREB1 Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the CREB1 antibody detected the CREB1 protein (arrow).
Application
CREB1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of CREB1 Antibody (Center)(Cat#AP11707c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
