Application
Western blot analysis of anti-CREBL2 Antibody (N-term K27) in CEM cell line lysates (35ug/lane). CREBL2 (arrow) was detected using the purified Pab.Application
Fluorescent confocal image of Hela cell stained with CREBL2 Antibody (N-term K27)(Cat#AP7441a). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CREBL2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CREBL2 immunoreactivity is localized to nucleus significantly.| Product Name | CREBL2 Antibody (N-term K27) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CREBL2; cAMP-responsive element-binding protein-like 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CREBL2 cAMP-responsive element-binding protein-like 2 |
|---|---|
| Molecular Weight(MW) | 13784 Da |
| Function | Probable regulator of CREB1 transcriptional activity which is involved in adipose cells differentiation. May also play a regulatory role in the cell cycle. Identification in a chromosomal region frequently deleted in various cancers suggests that it might act as a tumor suppressor |
| Tissue Specificity | This CREBL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18~48 amino acids from the N-terminal region of human CREBL2. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 1389 |
|---|
Application
Western blot analysis of anti-CREBL2 Antibody (N-term K27) in CEM cell line lysates (35ug/lane). CREBL2 (arrow) was detected using the purified Pab.
Application
Fluorescent confocal image of Hela cell stained with CREBL2 Antibody (N-term K27)(Cat#AP7441a). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CREBL2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CREBL2 immunoreactivity is localized to nucleus significantly.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
