Application
Western blot analysis of CRK (arrow) using CRK Antibody (S41) (RB17691). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CRK gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CRK Antibody (S41) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CRK Antibody (S41) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CRK Adapter molecule crk Proto-oncogene c-Crk p38 |
|---|---|
| Molecular Weight(MW) | 33831 Da |
| Function | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling |
| Tissue Specificity | This CRK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S41 of human CRK. |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Note=Translocated to the plasma membrane upon cell adhesion (By similarity) |
| Entrez Gene | 1398 |
|---|
Application
Western blot analysis of CRK (arrow) using CRK Antibody (S41) (RB17691). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CRK gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CRK Antibody (S41) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
