Application
A. Western blot analysis on HepG2 cell lysate using anti-Crk RabMAb (Cat. #AJ1201a).Application
B. Immunofluorescent staining of HeLa cells using anti-CRK RabMAb (Cat. #AJ1201a).| Product Name | Crk/p38 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRK, Proto-oncogene C-crk, p38;Adapter molecule crk |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP242Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:500~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CRK Proto-oncogene C-crk p38 Adapter molecule crk |
|---|---|
| Molecular Weight(MW) | 33831 Da |
| Function | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling |
| Tissue Specificity | A synthetic peptide corresponding to residues in the second SH3 domain and near C-terminus of human Crk (p38) protein was used as immunogen. |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Note=Translocated to the plasma membrane upon cell adhesion (By similarity) |
| Entrez Gene | 1398 |
|---|
Application
A. Western blot analysis on HepG2 cell lysate using anti-Crk RabMAb (Cat. #AJ1201a).
Application
B. Immunofluorescent staining of HeLa cells using anti-CRK RabMAb (Cat. #AJ1201a).| Application Notes | WB:1:500~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
