Application
A. Western blot analysis on K562 cell lysates using anti-Phospho-Crk/p38 (pY221) RabMAb (Cat. #AJ1201b), 1:2000 dilution. Cells were either (A) untreated (B) treated with AP.| Product Name | Crk/p38 Antibody Phospho (pY221) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRK, Proto-oncogene C-crk, p38;Adapter molecule crk |
| Modification | p-Y221 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR269Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CRK Proto-oncogene C-crk p38 Adapter molecule crk |
|---|---|
| Molecular Weight(MW) | 33831 Da |
| Function | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling |
| Tissue Specificity | A phospho specific peptide corresponding to regions surrounding Tyrosine 221 was used as an immunogen. This antibody detects proteins phosphorylated at Y221. |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Note=Translocated to the plasma membrane upon cell adhesion (By similarity) |
| Entrez Gene | 1398 |
|---|
Application
A. Western blot analysis on K562 cell lysates using anti-Phospho-Crk/p38 (pY221) RabMAb (Cat. #AJ1201b), 1:2000 dilution. Cells were either (A) untreated (B) treated with AP.| Application Notes | WB:1:1000~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
