Application
Western blot analysis of CRNN Antibody (Center) in K562 cell line lysates (35ug/lane). CRNN (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CRNN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CRNN Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRNN; C1orf10; DRC1; PDRC1; SEP53; Cornulin; 53 kDa putative calcium-binding protein; 53 kDa squamous epithelial-induced stress protein; 58 kDa heat shock protein; Squamous epithelial heat shock protein 53; Tumor-related protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C1orf10 DRC1 PDRC1 SEP53 |
|---|---|
| Alternative Names | CRNN C1orf10 DRC1 PDRC1 SEP53 Cornulin 53 kDa putative calcium-binding protein 53 kDa squamous epithelial-induced stress protein 58 kDa heat shock protein Squamous epithelial heat shock protein 53 Tumor-related protein |
| Molecular Weight(MW) | 53533 Da |
| Function | Survival factor that participates in the clonogenicity of squamous esophageal epithelium cell lines, attenuates deoxycholic acid (DCA)-induced apoptotic cell death and release of calcium. When overexpressed in oral squamous carcinom cell lines, regulates negatively cell proliferation by the induction of G1 arrest |
| Tissue Specificity | Squamous epithelia cell-specific. Expressed in the esophagus (periphery of the cells of the granular and the upper spinous layers), foreskin (granular and lower cornified cells), scalp skin (granular layer), inner root sheath of the hair follicle and in primary keratinocytes (at protein level) Expressed in the squamous epithelium of the cervix, esophagus, foreskin and larynx. Expressed in the fetal bladder and scalp skin. Expressed at very low levels in the lung, kidney, uterus, skeletal muscle, heart and fetal brain. Undetectable or barely detectable in esophageal and oral squamous cell carcinoma compared with the matched adjacent normal esophageal mucosa. Undetectable or barely detectable in larynx and esophagus from patients with pH-documented laryngopharyngeal reflux (LPR) |
| Cellular Localization | Cytoplasm. Note=Does not colocalize with TGM1 |
| Entrez Gene | 49860 |
|---|
Application
Western blot analysis of CRNN Antibody (Center) in K562 cell line lysates (35ug/lane). CRNN (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CRNN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
