Application
Western blot analysis of CRTC3 antibody (C-term) in MB435 cell line lysates (35ug/lane). CRTC3 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of 293 cells using CRTC3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
CRTC3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRTC3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | CRTC3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRTC3; TORC3; CREB-regulated transcription coactivator 3; Transducer of regulated cAMP response element-binding protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIHC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TORC3 |
|---|---|
| Alternative Names | CRTC3 TORC3 CREB-regulated transcription coactivator 3 Transducer of regulated cAMP response element-binding protein 3 |
| Molecular Weight(MW) | 66959 Da |
| Function | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR) |
| Tissue Specificity | Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach |
| Cellular Localization | Nucleus. Cytoplasm. Note=Appears to be mainly nuclear |
| Entrez Gene | 64784 |
|---|
Application
Western blot analysis of CRTC3 antibody (C-term) in MB435 cell line lysates (35ug/lane). CRTC3 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of 293 cells using CRTC3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
CRTC3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRTC3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
