Application
Western blot analysis of CRYZ Antibody (N-term) in K562, HepG2 cell line lysates (35ug/lane). CRYZ (arrow) was detected using the purified Pab.Application
CRYZ Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRYZ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CRYZ Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CRYZ Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRYZ; Quinone oxidoreductase; NADPH:quinone reductase; Zeta-crystallin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CRYZ Quinone oxidoreductase NADPH:quinone reductase Zeta-crystallin |
|---|---|
| Molecular Weight(MW) | 35207 Da |
| Function | Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding |
| Tissue Specificity | Only very low amounts in the lens. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 1429 |
|---|
Application
Western blot analysis of CRYZ Antibody (N-term) in K562, HepG2 cell line lysates (35ug/lane). CRYZ (arrow) was detected using the purified Pab.
Application
CRYZ Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRYZ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CRYZ Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
