Application
CSF2 Antibody (Center) western blot analysis in HL60 cell line lysates (35ug/lane).This demonstrates the CSF2 antibody detected the CSF2 protein (arrow).Application
CSF2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CSF2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CSF2 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of CSF2 Antibody (Center) (Cat#AP10690c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CSF2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSF2; GMCSF; Granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; Molgramostin; Sargramostim |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GMCSF |
|---|---|
| Alternative Names | CSF2 GMCSF Granulocyte-macrophage colony-stimulating factor Colony-stimulating factor Molgramostin Sargramostim |
| Molecular Weight(MW) | 16295 Da |
| Function | Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes |
| Tissue Specificity | This CSF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human CSF2. |
| Cellular Localization | Secreted. |
| Entrez Gene | 1437 |
|---|---|
| Nucleotide Accession | NP_000749.2 |
Application
CSF2 Antibody (Center) western blot analysis in HL60 cell line lysates (35ug/lane).This demonstrates the CSF2 antibody detected the CSF2 protein (arrow).
Application
CSF2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CSF2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CSF2 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of CSF2 Antibody (Center) (Cat#AP10690c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
