Application
A. Western blot analysis using anti-Casein Kinase II alpha subunit RabMAb on HeLa cell lysate (Cat. #AJ1128a), dilution 1:1000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Casein Kinase II alpha subunit RabMAb (Cat. #AJ1128a)| Product Name | CSNK2A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSNK2A1, CK2A1, Casein kinase II subunit alpha |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1963Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CK2A1 |
|---|---|
| Alternative Names | CSNK2A1 CK2A1 Casein kinase II subunit alpha |
| Molecular Weight(MW) | 45144 Da |
| Function | Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminal of human CKII protein was used as an immunogen. |
| Entrez Gene | 1457 |
|---|
Application
A. Western blot analysis using anti-Casein Kinase II alpha subunit RabMAb on HeLa cell lysate (Cat. #AJ1128a), dilution 1:1000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Casein Kinase II alpha subunit RabMAb (Cat. #AJ1128a)| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
