Application
Western blot analysis of CTLA4 Antibody (C-term) in K562 cell line lysates (35ug/lane). CTLA4 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of CTLA4 Antibody (C-term)(Cat#AP9453b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CTLA4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTLA4; CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CD_antigen=CD152; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD152 |
|---|---|
| Alternative Names | CTLA4 CD152 Cytotoxic T-lymphocyte protein 4 Cytotoxic T-lymphocyte-associated antigen 4 CD_antigen=CD152 Flags: Precursor |
| Molecular Weight(MW) | 24656 Da |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 |
| Tissue Specificity | Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation; |
| Entrez Gene | 1493 |
|---|
Application
Western blot analysis of CTLA4 Antibody (C-term) in K562 cell line lysates (35ug/lane). CTLA4 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of CTLA4 Antibody (C-term)(Cat#AP9453b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
