Application
Western blot analysis of CTNA1 Antibody (C-term) in Hela,MCF7,A2058 cell line lysates(35ug/lane). CTNA1 (arrow) was detected using the purified Pab.Application
CTNA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CTNA1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTNNA1; Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CTNNA1 Catenin alpha-1 Alpha E-catenin Cadherin-associated protein Renal carcinoma antigen NY-REN-13 |
|---|---|
| Molecular Weight(MW) | 100071 Da |
| Function | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments May play a crucial role in cell differentiation |
| Tissue Specificity | Expressed ubiquitously in normal tissues. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries |
| Entrez Gene | 1495 |
|---|
Application
Western blot analysis of CTNA1 Antibody (C-term) in Hela,MCF7,A2058 cell line lysates(35ug/lane). CTNA1 (arrow) was detected using the purified Pab.
Application
CTNA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
