Application
Western blot analysis of CTSF Antibody (Center Y194) in NCI-H460 cell line lysates (35ug/lane). CTSF (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human prostate tissue with CTSF Antibody (Center Y194), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using CTSF Antibody (Center Y194)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CTSF Antibody (Center Y194) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTSF; Cathepsin F |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CTSF Cathepsin F |
|---|---|
| Molecular Weight(MW) | 53366 Da |
| Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis |
| Tissue Specificity | High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus |
| Cellular Localization | Lysosome. |
| Entrez Gene | 8722 |
|---|
Application
Western blot analysis of CTSF Antibody (Center Y194) in NCI-H460 cell line lysates (35ug/lane). CTSF (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human prostate tissue with CTSF Antibody (Center Y194), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using CTSF Antibody (Center Y194)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
