Application
CUL2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the CUL2 antibody detected the CUL2 protein (arrow).Application
CUL2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CUL2 antibody detected the CUL2 protein (arrow).| Product Name | CUL2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUL2; Cullin-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CUL2 Cullin-2 |
|---|---|
| Molecular Weight(MW) | 86983 Da |
| Function | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) |
| Tissue Specificity | This CUL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-179 amino acids from the N-terminal region of human CUL2. |
| Entrez Gene | 8453 |
|---|---|
| Nucleotide Accession | NP_003582.2 |
Application
CUL2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the CUL2 antibody detected the CUL2 protein (arrow).
Application
CUL2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CUL2 antibody detected the CUL2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
