Application
A.Western blot analysis on (A) HeLa, (B) MCF-7, (C) A549 and (D) PC-12 cell lysates using anti-CUL1 RabMAb (Cat. #AJ1205b), dilution 1:5,000.Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-CUL1 RABMab (Cat. #AJ1205b).| Product Name | CUL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUL1, Cullin-1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3103Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CUL1 Cullin-1 |
|---|---|
| Molecular Weight(MW) | 89679 Da |
| Function | Core component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5 SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110 (By similarity) |
| Tissue Specificity | Expressed in lung fibroblasts. |
| Entrez Gene | 8454 |
|---|
Application
A.Western blot analysis on (A) HeLa, (B) MCF-7, (C) A549 and (D) PC-12 cell lysates using anti-CUL1 RabMAb (Cat. #AJ1205b), dilution 1:5,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-CUL1 RABMab (Cat. #AJ1205b).| Application Notes | WB:1:2000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
