Application
A. Western blot analysis on (A) HeLa, (B) SH-SY5Y, (C) PC12 and (D) NIH/3T3 cell lysates using anti-CUL3 RabMAb (Cat. #AJ1206b), dilution 1:5000.| Product Name | CUL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUL3, KIAA0617, Cullin-3 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3195 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0617 |
|---|---|
| Alternative Names | CUL3 KIAA0617 Cullin-3 |
| Molecular Weight(MW) | 88930 Da |
| Function | Core component of multiple cullin-RING-based BCR (BTB- CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(KLHL42) is involved in ubiquitination of KATNA1. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3 BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; possibly by mediating ubiquitination of SLC12A3/NCC Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41 |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus. Golgi apparatus. |
| Entrez Gene | 8452 |
|---|
Application
A. Western blot analysis on (A) HeLa, (B) SH-SY5Y, (C) PC12 and (D) NIH/3T3 cell lysates using anti-CUL3 RabMAb (Cat. #AJ1206b), dilution 1:5000.| Application Notes | WB:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
