Application
Western blot analysis of CYB5R1 Antibody (C-term) in MDA-MB435, MCF-7 cell line lysates (35ug/lane). CYB5R1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CYB5R1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CYB5R1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CYB5R1 (arrow) using rabbit polyclonal CYB5R1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5R1 gene.| Product Name | CYB5R1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYB5R1; NQO3A2; NADH-cytochrome b5 reductase 1; Humb5R2; NAD(P)H:quinone oxidoreductase type 3 polypeptide A2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NQO3A2 |
|---|---|
| Alternative Names | CYB5R1 NQO3A2 NADH-cytochrome b5 reductase 1 Humb5R2 NAD(P)H:quinone oxidoreductase type 3 polypeptide A2 |
| Molecular Weight(MW) | 34095 Da |
| Function | NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity) |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Membrane; Single-pass membrane protein (Potential) |
| Entrez Gene | 51706 |
|---|
Application
Western blot analysis of CYB5R1 Antibody (C-term) in MDA-MB435, MCF-7 cell line lysates (35ug/lane). CYB5R1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CYB5R1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CYB5R1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CYB5R1 (arrow) using rabbit polyclonal CYB5R1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5R1 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
