Application
CYBA Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CYBA antibody detected the CYBA protein (arrow).Application
Confocal immunofluorescent analysis of CYBA Antibody (C-term)(Cat#AP12149b) with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | CYBA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYBA; Cytochrome b-245 light chain; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CYBA Cytochrome b-245 light chain Cytochrome b(558) alpha chain Cytochrome b558 subunit alpha Neutrophil cytochrome b 22 kDa polypeptide Superoxide-generating NADPH oxidase light chain subunit p22 phagocyte B-cytochrome p22-phox |
|---|---|
| Molecular Weight(MW) | 21013 Da |
| Function | Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide |
| Tissue Specificity | This CYBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-153 amino acids from the C-terminal region of human CYBA. |
| Cellular Localization | Cell membrane. |
| Entrez Gene | 1535 |
|---|---|
| Nucleotide Accession | NP_000092.2 |
Application
CYBA Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CYBA antibody detected the CYBA protein (arrow).
Application
Confocal immunofluorescent analysis of CYBA Antibody (C-term)(Cat#AP12149b) with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
