Application
A. Western blotting assay using MCF-7 cell lysate with Rabbit anti-Cyclin D1 monoclonal antibody (Cat. #AJ1209c), 1:5000 dilutionApplication
B. Immunohistochemical staining of paraffin-embedded Human mantle cell lymphoma using anti-Cyclin D1RabMAb (Cat. #AJ1209c).| Product Name | Cyclin D1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCND1, BCL1, PRAD1, G1/S-specific cyclin-D1, PRAD1 oncogene;BCL-1 oncogene |
| Clonality | Monoclonal |
|---|---|
| Clone Number | SP4 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCL1 PRAD1 |
|---|---|
| Alternative Names | CCND1 BCL1 PRAD1 G1/S-specific cyclin-D1 PRAD1 oncogene BCL-1 oncogene |
| Molecular Weight(MW) | 33729 Da |
| Function | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex |
| Tissue Specificity | A synthetic peptide from C-terminus of human cyclin D1. |
| Cellular Localization | Nucleus. Cytoplasm. Membrane. Note=Cyclin D- CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members (By similarity) |
| Entrez Gene | 595 |
|---|
Application
A. Western blotting assay using MCF-7 cell lysate with Rabbit anti-Cyclin D1 monoclonal antibody (Cat. #AJ1209c), 1:5000 dilution
Application
B. Immunohistochemical staining of paraffin-embedded Human mantle cell lymphoma using anti-Cyclin D1RabMAb (Cat. #AJ1209c).| Application Notes | WB:1:5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
