Application
Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35ug/lane). Cyclin D1(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Formalin-fixed and paraffin-embedded human Testis tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90)(Cat#AP2612d) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Product Name | Cyclin D1 Antibody (S90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1 oncogene; PRAD1 oncogene |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCL1 PRAD1 |
|---|---|
| Alternative Names | CCND1 BCL1 PRAD1 G1/S-specific cyclin-D1 B-cell lymphoma 1 protein BCL-1 oncogene PRAD1 oncogene |
| Molecular Weight(MW) | 33729 Da |
| Function | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex |
| Tissue Specificity | This Cyclin D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S90 of human Cyclin D1. |
| Cellular Localization | Nucleus. Cytoplasm. Membrane. Note=Cyclin D- CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members (By similarity) |
| Entrez Gene | 595 |
|---|
Application
Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35ug/lane). Cyclin D1(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Formalin-fixed and paraffin-embedded human Testis tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90)(Cat#AP2612d) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
