Application
CYC1 Antibody (C-term) western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).This demonstrates the CYC1 antibody detected the CYC1 protein (arrow).Application
CYC1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYC1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CYC1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CYC1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYC1; Cytochrome c1, heme protein, mitochondrial; Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CYC1 Cytochrome c1 heme protein mitochondrial Complex III subunit 4 Complex III subunit IV Cytochrome b-c1 complex subunit 4 Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit |
|---|---|
| Molecular Weight(MW) | 35422 Da |
| Function | This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain |
| Tissue Specificity | This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-297 amino acids from the C-terminal region of human CYC1. |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side |
| Entrez Gene | 1537 |
|---|---|
| Nucleotide Accession | NP_001907.2 |
Application
CYC1 Antibody (C-term) western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).This demonstrates the CYC1 antibody detected the CYC1 protein (arrow).
Application
CYC1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYC1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CYC1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
