Application
Western blot analysis on (A) Jurkat, (B) A431, (C) K562, and (D) HeLa cell lysates using anti-Cyclin H RabMAb (Cat. #AJ1213a).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclin H RabMAb (Cat. #AJ1213a).Application
Immunofluorescent staining of HeLa cells using anti-Cyclin H RabMAb (Cat. #AJ1213a).| Product Name | Cyclin H Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3929 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 37643 Da |
|---|---|
| Function | Regulates CDK7, the catalytic subunit of the CDK- activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle |
| Tissue Specificity | A synthetic peptide corresponding to residues in human Cyclin H was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 902 |
|---|
Application
Western blot analysis on (A) Jurkat, (B) A431, (C) K562, and (D) HeLa cell lysates using anti-Cyclin H RabMAb (Cat. #AJ1213a).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclin H RabMAb (Cat. #AJ1213a).
Application
Immunofluorescent staining of HeLa cells using anti-Cyclin H RabMAb (Cat. #AJ1213a).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
