Application
Western blot analysis of anti-CCNC Antibody (C-term) in HL60 cell line lysates (35ug/lane). CCNC(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | Cyclin C (CCNC) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCNC; Cyclin-C; SRB11 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CCNC Cyclin-C SRB11 homolog |
|---|---|
| Molecular Weight(MW) | 33243 Da |
| Function | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex |
| Tissue Specificity | Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 892 |
|---|
Application
Western blot analysis of anti-CCNC Antibody (C-term) in HL60 cell line lysates (35ug/lane). CCNC(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
