Application
Western blot analysis of CYP2A13 Antibody (Center) pre-incubated without(lane 1) and with(lane 2) blocking peptide in MDA-MB435 cell line lysate. CYP2A13 (arrow) was detected using the purified Pab (1:60 dilution).Application
CYP2A13 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYP2A13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CYP2A13 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CYP2A13 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYP2A13; Cytochrome P450 2A13; CYPIIA13 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CYP2A13 Cytochrome P450 2A13 CYPIIA13 |
|---|---|
| Molecular Weight(MW) | 56688 Da |
| Function | Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N- dimethylaniline, 2'-methoxyacetophenone, N- nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4- (methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity |
| Tissue Specificity | Expressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein |
| Entrez Gene | 1553 |
|---|
Application
Western blot analysis of CYP2A13 Antibody (Center) pre-incubated without(lane 1) and with(lane 2) blocking peptide in MDA-MB435 cell line lysate. CYP2A13 (arrow) was detected using the purified Pab (1:60 dilution).
Application
CYP2A13 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYP2A13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CYP2A13 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
