Application
Western blot analysis of anti-CYP27A1 Antibody (N-term) in K562 cell line lysates (35ug/lane). CYP27A1(arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of CYP27A1 Antibody (N-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CYP27A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYP27A1; CYP27; Sterol 26-hydroxylase, mitochondrial; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYP27 |
|---|---|
| Alternative Names | CYP27A1 CYP27 Sterol 26-hydroxylase mitochondrial 5-beta-cholestane-3-alpha 7-alpha 12-alpha-triol 27-hydroxylase Cytochrome P-450C27/25 Cytochrome P450 27 Sterol 27-hydroxylase Vitamin D(3) 25-hydroxylase |
| Molecular Weight(MW) | 60235 Da |
| Function | Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity |
| Tissue Specificity | This CYP27A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110~140 amino acids from the N-terminal region of human CYP27A1. |
| Cellular Localization | Mitochondrion membrane. |
| Entrez Gene | 1593 |
|---|
Application
Western blot analysis of anti-CYP27A1 Antibody (N-term) in K562 cell line lysates (35ug/lane). CYP27A1(arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of CYP27A1 Antibody (N-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
