Application
Western blot analysis of anti-CYP39A1 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). CYP39A1 was detected using the purified Pab .Application
CYP39A1 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of CYP39A1 Antibody (N-term)(Cat#AP2824a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
CYP39A1 Antibody (N-term) (AP2824a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP39A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | CYP39A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYP39A1; 24-hydroxycholesterol 7-alpha-hydroxylase; Cytochrome P450 39A1; Oxysterol 7-alpha-hydroxylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIFIHC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CYP39A1 24-hydroxycholesterol 7-alpha-hydroxylase Cytochrome P450 39A1 Oxysterol 7-alpha-hydroxylase |
|---|---|
| Molecular Weight(MW) | 54116 Da |
| Function | Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha- hydroxylated product |
| Tissue Specificity | Liver specific. |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein |
| Entrez Gene | 51302 |
|---|
Application
Western blot analysis of anti-CYP39A1 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). CYP39A1 was detected using the purified Pab .
Application
CYP39A1 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of CYP39A1 Antibody (N-term)(Cat#AP2824a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
CYP39A1 Antibody (N-term) (AP2824a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP39A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
