Application
Western blot analysis of CYP2J2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). CYP2J2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP2J2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using CYP2J2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CYP2J2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYP2J2; Cytochrome P450 2J2; Arachidonic acid epoxygenase; CYPIIJ2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CYP2J2 Cytochrome P450 2J2 Arachidonic acid epoxygenase CYPIIJ2 |
|---|---|
| Molecular Weight(MW) | 57611 Da |
| Function | This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools |
| Tissue Specificity | Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein |
| Entrez Gene | 1573 |
|---|
Application
Western blot analysis of CYP2J2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). CYP2J2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP2J2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using CYP2J2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
