Application
Western blot analysis of anti-CYP7A1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CYP7A1 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of CYP7A1 Antibody (N-term)(Cat#AP7996b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | CYP7A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYP7A1; CYP7; Cholesterol 7-alpha-monooxygenase; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYP7 |
|---|---|
| Alternative Names | CYP7A1 CYP7 Cholesterol 7-alpha-monooxygenase CYPVII Cholesterol 7-alpha-hydroxylase Cytochrome P450 7A1 |
| Molecular Weight(MW) | 57661 Da |
| Function | Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis |
| Tissue Specificity | Detected in liver. |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein |
| Entrez Gene | 1581 |
|---|
Application
Western blot analysis of anti-CYP7A1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CYP7A1 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of CYP7A1 Antibody (N-term)(Cat#AP7996b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
