Application
A. Western blot analysis on A431 cell lysate using anti-CK-14 RabMAb (Cat. #AJ1219a), dilution 1:20,000| Product Name | Cytokeratin-14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT14, Keratin, type I cytoskeletal 14, Keratin-14 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1612Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:20000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | KRT14 Keratin type I cytoskeletal 14 Keratin-14 |
|---|---|
| Molecular Weight(MW) | 51561 Da |
| Function | The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro |
| Tissue Specificity | Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen |
| Cellular Localization | Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern |
| Entrez Gene | 3861 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-CK-14 RabMAb (Cat. #AJ1219a), dilution 1:20,000| Application Notes | WB:1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
